Are you sure spi works?
Apart from that, make sure the stm32 and the eeprom are in sync, that is, they both come from the same saving of "Save all files"
and that the firmware is compiled and downloaded. It doesn't hurt to do it one more time.
Power cycle the board to make sure both stm32 and lan9252 read the most recent data.
Right-click the mouse on the marked item and remove it. Scan for new boxes to make it reappear.
Also quit Twincat and start it.
All a bit extra cautious to make sure any old data/cache is eliminated.
