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Chromium has nothing to do with no direction change.
Check wiring first, most probably loose wire inside a connector or broken wire.

I have used 2.7.14 for several years now without problems.  Friday, Chromium stopped working so I reinstalled it.  Now _ and - both go the same direction.  So I reinstalled. No change.  Suggestions?  Sherline mill.

that is because this is wrong
and this does nothing no matter what value

did your setup work before with mach3?

if it is stepper timing related you need to load the DMA stepgen config and adjust your step timing accordingly